In order to determine the inoculation time of the microorganisms,
general factorial design was used. Factors were designated as
inoculation time of T.harzianum, A.sojae and S.cerevisae with five
levels (0 h, 24 h, 48 h, 72 h and 96 h). In order to make the experiments
more practical, designs were separated into two parts.
For the first design, T.harzianum was inoculated into the flasks at
0th h. The other two organisms were combined with each other.
And for the second design, A.sojae was inoculated into the flasks at
the beginning and remaining organisms were combined with each
other. Total of 50 experiments were conducted with 2 replicas of
each factorial combinations for both designs.