Animals and offspring were counted and exposure was exchanged
every second day. M. macrocopa was fed with the green algae
Pseudokirchneriella subcapitata (Koršikov) Hindák. P. subcapitata
strain NIVA-CHL 1 was obtained from the Culture Collection of Algae
at the University of Göttingen, Germany. The algae were cultivated
in freshwater medium (Nicklisch et al., 2008), and grown in batch
cultures under day light conditions. The animals were fed daily ad
libitum with 10 mLof a logarithmically growing culture which corresponds
to 107 cells or approximately 2.5 mg L1 C