Aliquots (2 mL) of cell suspension, treated with or without
microbial growth inhibitors from lignocellulosic feedstocks, were
centrifuged at 8000g for 15 min, and the supernatants were
removed completely. In the case of lipid extraction from dried cell
mass, the pellets were lyophilized. The moisture content of the
wet cell mass pellets was 67.0 (±3.3)% as determined by measuring
the mass differences before and after lyophilization. Each
extraction was carried out in 15-ml glass tubes with screw caps
on a rotary shaker at 150 rpm (Multitron, Infors, Bottmingen,
Switzerland). The dried cell mass (approximately 22 mg) or the
wet cell mass (approximately 67.3 mg, equivalent to 22 mg of
dried cell mass) was mixed with 2 mL of the desired organic solvent
at appropriate temperatures. After incubation for designated
Aliquots (2 mL) of cell suspension, treated with or withoutmicrobial growth inhibitors from lignocellulosic feedstocks, werecentrifuged at 8000g for 15 min, and the supernatants wereremoved completely. In the case of lipid extraction from dried cellmass, the pellets were lyophilized. The moisture content of thewet cell mass pellets was 67.0 (±3.3)% as determined by measuringthe mass differences before and after lyophilization. Eachextraction was carried out in 15-ml glass tubes with screw capson a rotary shaker at 150 rpm (Multitron, Infors, Bottmingen,Switzerland). The dried cell mass (approximately 22 mg) or thewet cell mass (approximately 67.3 mg, equivalent to 22 mg ofdried cell mass) was mixed with 2 mL of the desired organic solventat appropriate temperatures. After incubation for designated
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