Samples of 4 mg each
were weighed and dissolved in 3.9 mL of distilled water. Subsequently,
4.1 mL of 2.52% linoleic acid in absolute ethanol, 8 mL of
0.05 M phosphate buffer (pH 7.0) and 3.9 mL of distilled water
were added and placed in an oven at 40 C in the dark. To a
0.1 mL aliquot of this solution, 9.7 mL of 75% ethanol and 0.1 mL
of 30% ammonium thiocyanate were added.