Finally, 1000 mL of media volume was
used for bulk culturing and cells were harvested at stationary
growth phase by centrifuging at 2200 × g for 15 min at 5 °C.
The supernatant was discarded and the precipitated cell mass
was washed twice with sterile peptone water (0.2%, w/v). The
final wet cell mass was weighed and divided into two equal
portions. One portion (wet mass 5 g) was used to prepareWPIP-
GA microcapsules and the second half (wet mass 5 g) was
used to prepare WPI-P-O-GA co-microcapsules in order to facilitate
a good comparative study