Ambulances may be a source of multidrug-resistant microorganisms (MDROs) because patient microbiota may colonize health care personnel and an ambulance’s environment during their assistance. Contaminated hands are main sources of microbial transmission causing health care-associated infections.1,2 Basic life support ambulances (BLSAs) link the community and health care facilities and a lack of basic infection control measures could promote the exchange of MDROs.3-5 This study aimed to analyze microbial contamination and antimicrobial resistance profiles of clinically relevant microorganisms isolated from BLSAs.