2.3. Isolation and identification of bacteria from fish
Briefly, swabs from individual fish were taken from the eye, brain and kidney, and streaked directly onto Tryptic Soy Agar (Merck, Darmstadt, Germany) with 5% human blood. The agars were then incubated for 24 h at 37 °C. The fish were handled and sacrificed according to method approved by Animal Utilization Protocol, Faculty of Veterinary Medicine, Universiti Putra Malaysia.