The position of each ‘G’ genes defined by the first and the second axes were generated by COA on RSCU values as shown in Fig. 1. The first principle axis accounted for 25% of the total variation and the rest all (second, third and fourth) axes code for equal variations i.e. 25 %. Therefore, both the axes have an impact on the variations of synonymous codon usage of the ‘G’ gene.