ิิbecause the film does not come into contact with food and, consequently, the bacteriophage is not in contact with the bacteria. The in vitro test was performed without the film being in contact with food to represent the same conditions. The release to the environment occurred only during the analysis process, when the film was stored during this time (14 days). As previous studies have shown that encapsulation of phages in various biopolymer matrices lead to significant loss in activity. The dried microspheres with encapsulated phages were combined with trehalose, a common stabilizing agent for viruses, to increase the phage stability after drying (Bieganski, Fowler, Morgan, & Toner, 1998). Similar encapsulation of phages in synthetic PVA (polyvinyl alcohol) polymer using the electrospinning process has shown a significant loss in number of viable phages (∼1e 6% viable phages) immediately after electrospinning