2.3. Endophytic colonization experiments
To quantify the endophytic colonization of different T. stromaticum isolates, five cacao and bean plants treated with each of the isolates used in this study were removed from the sterile test tubes or from the pots one month after the inoculations. Cacao and bean plants were divided into roots, crown, stem, cotyledon, leaves, and shoot tips. Cotyledons of bean plants were not evaluated in the nonsterile system because they detached early and were decomposed by saprotrophs. Each of these parts was disinfested according to the methodology described by Petrini et al. (1989) and then subdivided into 3–15 pieces to estimate the percentage of colonization, plated onto 1/5 strength PDA and incubated at room temperature.
2.3. Endophytic colonization experimentsTo quantify the endophytic colonization of different T. stromaticum isolates, five cacao and bean plants treated with each of the isolates used in this study were removed from the sterile test tubes or from the pots one month after the inoculations. Cacao and bean plants were divided into roots, crown, stem, cotyledon, leaves, and shoot tips. Cotyledons of bean plants were not evaluated in the nonsterile system because they detached early and were decomposed by saprotrophs. Each of these parts was disinfested according to the methodology described by Petrini et al. (1989) and then subdivided into 3–15 pieces to estimate the percentage of colonization, plated onto 1/5 strength PDA and incubated at room temperature.
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