, from Figure 3-B will be that when we pay attention to the supplement. Over time, make it Escherichia coli has the value Ethanol increased by repeated time and cost more than Klebsiella oxytoca Ethanol, until over time 100 hours Escherichia coli and Klebsiella oxytoca will have the same Ethanol. When 0.5% yeast extract Escherichia coli and Klebsiella oxytoca will have Ethanol increase, over time 60 hours Escherichia coli and Klebsiella oxytoca are not constant, over time 100 hours Escherichia coli and Klebsiella oxytoca will have the same value. When put 0.5% yeast extract, thiamine and micronutrients Escherichia coli have Ethanol increased consistently over time 80 hours Escherichia coli is constant. The Klebsiella oxytoca have Ethanol increased regularly. When the time 60 hours Klebsiella oxytoca is not constant.