The pure culture of the Trichoderma was isolated from
individual carrier materials at an interval of three months
by means of pour plating till the end of the incubation
period and the antagonistic potential against S. rolfsii
was determined by dual culture method (Skidmore and
Dickinson, 1976) and compared with the inhibition given
by the fresh culture of the same strain. The percentage
inhibition of mycelia growth of pathogen was worked
out as follows: