In order to solve insufficiency of information content and resolution with chlorophyll fluorescence spectrometry, a method of LED-induced chlorophyll fluorescence analysis fused image processing technique is presented. Using small, long life and high-intensity blue (460nm) LED lamp for the near field detection, plants leaf fluorescence is induced actively based on the theory of active-inducing chlorophyll fluorescence. The method of extracting chlorophyll fluorescence characteristics is studied, by using spectrometer and image sensor fixed filter to obtain spectraand image information respectively.Through analyzing chlorophyll fluorescence spectra data and image data, the results indicate that fluorescence information and chlorophyll content have strong correlation. This research lays the foundation for living body real-time online measurement in situ.