2.7. Carotenoid and lycopene content
HPLC analysis of _-carotene and lycopene contents was carried out with the Agilent 1100 Series system, Waldborn Gemany (pump, detector, software).
The LC column Zorbax-Eclipse XDBC18; 4.6 mm ×250 mm, 5 _m was used, with
a mobile phase consisting of a mixture of acetonitrile:methanol:ethyl acetate, at
a flow rate of 1 cm3/min. The injection volume was 20 _l using the detector DAD Agilent 1200 Series at 470 nm wavelength (Cvetkovic and Markovic, 2008).