Chlorophyll development seems to be controlled
by many genes located near the centromere and
proximal segments of the chromosomes [20].
Localized chromosome breakage by EMS treatment
as reported by might be the reason of getting
chlorophyll deficient mutants in EMS treatments [21].
The frequency of chlorophyll mutation in M2 generation
is generally regarded as the most reliable index of
mutation rate because of greater accuracy of scoring
however, in the present investigation it indicated neither
the spectrum of mutation nor the probability of isolating
promising macro-mutants.