The dissected ovarian lobes were fixed in 10% buffered formalin solution for 11 h., dehydrated in 70% alcohol and lastly placed in tissue processor for 18 ± 1 h at 60 °C. After processing, samples were mounted onto their cassettes using paraffin wax, sectioned into 5μm films using a microtome (Leica RM835), transferred into water bath (between 40 and 45 °C) for expansion before mounting onto slides (glycerol + egg white was used as adhesive).