Following sample collection, tissues were homogenized in 0.1 M perchloric acid by manual sonication, centrifuged at 10,000 rpm for 20 min to remove supernatant and stored at −80 ◦C until the determination of monoamine levels.
Following sample collection, tissues were homogenized in 0.1 M perchloric acid by manual sonication, centrifuged at 10,000 rpm for 20 min to remove supernatant and stored at −80 ◦C until the determination of monoamine levels.