Before catheterization the local anesthesia (Xylocaine®) is given and after removal of
catheter the animal was treated with antibiotic (Oxytetracycline) for 3 days. The blood samples were collected in
heparinized polypropylene tubes and immediately kept in icebox (4 °C) and then centrifuged at 3000 rpm for 20 min at 4 °C.
Plasma separated out was stored at −20 °C till assayed for GH.