Currently there are six Persea taxathat are listed as threatened on the Red List of International Unionfor Conservation of Nature (Muriel and Pitman, 2004). This exem-plifies the urgent need for a long-term germplasm conservationmethod that is not exposed to the vagaries of climate change anddisease and pest incursions (Barrientos-Priego, 1999; Ben-Ya’acovand Barrientos-Priego, 2003).