Effect of the defatting procedures and
the protein isolation process on the
thermal properties of the SPIs
Table 4 shows the effect of the defatting procedures and the
protein isolation process on the thermal properties of the SPI. In
general, the SPI samples all exhibited 2 thermal transitions at approximately
75 °C and 93 °C corresponding to the denaturation
temperature of -conglycinin (7S) and glycinin (11S), respectively.
These values are similar to those reported by other workers (Arrese
and others 1991; Wagner and others 1996; Riblett and others 2001).
The denaturation temperatures of glycinin and -conglycinin were,
thus, not altered by both alternative defatting and protein extraction
processes. However, both the enthalpy and the peak width (T1/2)