Furthermore, the application of edible films as growth inhibitors of microbial spoilage and pathogens has been also reported.Regarding probiotic edible films, López de Lacey et al. (2012) recently showed that the immobilisation of Bifidobacteria bifidum and Lactobacillus acidophilus in gelatine based matrices could provide a good protection against viability loss for at least six days of storage under chilled conditions e.g. 2 C and Kanmani and Lim (2013) reported that the viability of multiple probiotic strains e.g. Lactobacillus reuteri ATCC 55730, Lactobacillus plantarum GG ATCC
53103 and L. acidophilus DSM 20079 in starchepullulan based edible films was strongly influenced by the pullulan to starch ratio and storage temperature.