A bio-barcoded DNA assay utilizes oligonucleotide-modified gold nanoparticles (AuNPs) for signal amplification and magnetic nanoparticles (MNPs) for easy separation from the sample.
The large bDNA-to-recognition agent ratio provides a means of sub- stantial signal amplification.
It is promising as it allows the rapid detection of multiple protein targets at low-attomolar concentrations (Goluch et al., 2006)
and nucleic acids at high-zeptomolar levels under optimized conditions (Nam et al., 2004).