DNA was isolated from a material withdrawn from legs of investigated
insects. The Sherlock AX Purification Kit (A&A Biotechnology) was
used according to the manufacturer's instruction.
Fragments of genomic DNA, corresponding to particular tested
genes, were amplified by PCR with the use of primers which are listed
in Table 1. Amplified DNA was separated by agarose gel electrophoresis
and analyzed according to Sambrook and Russell (2001).