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Four bacterial strains of Bacillus spp. which were antagonistic to the mango anthracnose pathogen were iso- lated and screened. Among them, TB09 and TB72 were identified by 16S rDNA sequence as Bacillus pumilus and Bacillus thuringiensis, respectively. In vitro, the anthracnose fungus showed 88.87% and 80.07% of myce- lia growth inhibitions in presence of B. pumilus and B. thuringiensis, respectively and in vivo, the inhibitions of the disease were 94.28% and 87.06%, respectively. Based on the Gas Chromatography–Mass Spectrometer (GC–MS) analysis, 11 volatile compounds produced by B. pumilus and B. thuringiensis were identified. Among them, five volatiles showed better inhibition effects on the pathogens. 2-nonanone, b-benzenee- thanamine, 2-decanone completely inhibited mycelia growth in vitro at a concentration of 100 lL L