Capillary zone electrophoresis (CZE) with multi-wavelength detection was used for the separation of the main
betacyanins, betanin and isobetanin, and the corresponding aglycones from extracts of Beta vulgaris. Optimum
separation was achieved with a fused-silica capillary tube and a citric acid-NazHPO 4 buffer (10 mM). The applied
voltage was -22 kV and the capillary thermostating temperature was kept constant at 15°C. The results of the
quantitative CZE analyses were compared with those obtained by HPLC.