The cytotoxicity of complexes 1 and 2, was evaluated using a
SNO cancer cell line. The viability of the cells was determined using
an alamarBlue viability assay, after the 24 h treatment with complexes
1 and 2. This assay involves the incubation of the treated
cells with the alamarBlue dye for 2–3 h. The assay is based on
the detection of cellular proliferation. When the cells are viable,
they have a reducing environment in the cytosol. When the dye
is introduced and taken up by viable cells, the active compound
in the dye, resazurin (blue) will be reduced to resorufin (red).
The active product does not have any fluorescent properties; however,
the reduced product exhibits fluorescence properties which
can be measured spectrophotometrically at k 530 (excitation)/k
590 (emission) nm. The % viability can be measured accordingly.
The untreated control represents 100% viable cells. The fluorescence
of the treated cells will be calculated relative to the
untreated control. Statistical analysis was performed by using the
two tailed Student’s t-test to determine the significant decrease