Proteomics in conjunction with biochemical strategy was employed to unravel regulation of lipid metabolism
in the green microalga Chlorella protothecoides by heterotrophy–photoinduction cultivation regime
(HPC). Interestingly, HPC triggered transiently synthesis of starch followed by substantial lipid accumulation.
And a marked decrease in intracellular protein and chlorophyll contents was also observed after
12 h of photo-induction. The highest lipid content of 50.5% was achieved upon the photo-induction stage,
which represented 69.3% higher than that of the end of heterotrophic cultivation. Results suggested that
turnover of carbon–nitrogen-rich compounds such as starch, protein, and chlorophyll might provide carbon
or energy for lipid accumulation. The proteomics analysis indicated that several pathways including
glycolysis, TCA cycle, b-oxidation of fatty acids, Calvin cycle, photosynthesis, energy and transport, protein
biosynthesis, regulate and defense were involved in the lipid biosynthesis. Malate dehydrogenase
and acyl-CoA dehydrogenase were suggested as key regulatory factors in enhancing lipid accumulation.
2014 Elsevier Ltd. All rights reserved.
Proteomics in conjunction with biochemical strategy was employed to unravel regulation of lipid metabolismin the green microalga Chlorella protothecoides by heterotrophy–photoinduction cultivation regime(HPC). Interestingly, HPC triggered transiently synthesis of starch followed by substantial lipid accumulation.And a marked decrease in intracellular protein and chlorophyll contents was also observed after12 h of photo-induction. The highest lipid content of 50.5% was achieved upon the photo-induction stage,which represented 69.3% higher than that of the end of heterotrophic cultivation. Results suggested thatturnover of carbon–nitrogen-rich compounds such as starch, protein, and chlorophyll might provide carbonor energy for lipid accumulation. The proteomics analysis indicated that several pathways includingglycolysis, TCA cycle, b-oxidation of fatty acids, Calvin cycle, photosynthesis, energy and transport, proteinbiosynthesis, regulate and defense were involved in the lipid biosynthesis. Malate dehydrogenaseand acyl-CoA dehydrogenase were suggested as key regulatory factors in enhancing lipid accumulation. 2014 Elsevier Ltd. All rights reserved.
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