. 1350 bp
and 751 bp cDNA fragments from mycoviruses AbEV1 and AbFV1,
respectively, were amplified from the three strains (817-14, FY730-
7, and YY10-7), whereas no RT-PCR product was detected from
a dsRNA-free sample (Fig. 1b). Therefore, the three fungal strains
appear to be infected by the same mycoviral species.
In conclusion, we proved the presence of dsRNA with sizes ranging
from 2.5 kbp to 10 kbp in 5 strains in a collection of 25 A.
brassicicola strains. In addition, we have characterized the molecular
and biological properties of a novel mycovirus, named AbFV1
from A. brassicicola which was similar to the SsFV1 in family
Fusariviridae infecting Sclerotinia sclerotiorum. Based on genomic
organization and phylogenetic analysis, it can be ascertained that
AbFV1 is a (+ssRNA) mycovirus and represents a novel member of
family Fusariviridae. To the best of our knowledge, this is the first
report of a (+ssRNA) mycovirus infecting an A. brassicicola fungus