Fig. 1 Flow chart of a typical
FISH. The procedure includes
the following steps: (1) fixation
of the specimen directly in
sample tubes; (2) transfer of the
sample on a glass slide and
preparation of the sample, in-
cluding specific pretreatment
steps; (3) hybridization with the
respective probes for detecting
the respective target sequences;
(4) washing steps to remove
unbound probes; (5) mounting,
visualization and documentation
of results