Sucrose was then chosen as the carbon adjunct in the raw juice for the preparation high gravity (20 Brix containing 200 gl) and very high gravity (28 Brix containing 280 gl) media. In industrial scale, the juice extracted from many tons of the sweet sorghum stalks has to be kept in concentrated form (as molasses) to reduce storage area and the risk of bacterial contamination. To prepare high gravity or very high gravity medium for ethanol production, the concentrated juice (about 65-70 Brix containing about 650-700 gl of tatal sugar) must be diluted to the desired concentrations. Therefore, no sucrose is required for the preparation of high gravity and very high gravity media in the large scale.