[Column 8] Methylmercury Analysis in National Institute for Minamata Disease (NIMD)
Most biological samples, including human/blood and fish, contain both methylmercury (MeHg) and inorganic mercury (I-Hg). For exposure assessment and risk assessment, not only total mercury (MeHg + I-Hg) analysis but selective quantification of MeHg of the samples is necessary. The total mercury analysis can be successfully performed using an atomic absorption detector. For selective quantification of MeHg, an electron capture detector-gas chromatography (ECD-GC) is used after the extraction of MeHg to organic solvent such as toluene. However, it was difficult previously to obtain an exact analytical result due to incomplete extraction of MeHg using an organic solvent. The National Institute for Minamata Disease (NIMD) has established a novel technique to improve the extraction efficiency up to nearly 100% with the use of dithizone /toluene as a solvent. Currently, the NIMD provides MeHg analysis data of high quality in various samples, including environmental samples such as seawater and soil. The technique has been transferred to mercury scientists in such countries as Brazil, Tanzania, Nicaragua, Indonesia and Korea through international cooperation activities of the NIMD, and been used in MeHg analysis there. Detail of the method is available on the NIMD website
[Column 8] Methylmercury Analysis in National Institute for Minamata Disease (NIMD)Most biological samples, including human/blood and fish, contain both methylmercury (MeHg) and inorganic mercury (I-Hg). For exposure assessment and risk assessment, not only total mercury (MeHg + I-Hg) analysis but selective quantification of MeHg of the samples is necessary. The total mercury analysis can be successfully performed using an atomic absorption detector. For selective quantification of MeHg, an electron capture detector-gas chromatography (ECD-GC) is used after the extraction of MeHg to organic solvent such as toluene. However, it was difficult previously to obtain an exact analytical result due to incomplete extraction of MeHg using an organic solvent. The National Institute for Minamata Disease (NIMD) has established a novel technique to improve the extraction efficiency up to nearly 100% with the use of dithizone /toluene as a solvent. Currently, the NIMD provides MeHg analysis data of high quality in various samples, including environmental samples such as seawater and soil. The technique has been transferred to mercury scientists in such countries as Brazil, Tanzania, Nicaragua, Indonesia and Korea through international cooperation activities of the NIMD, and been used in MeHg analysis there. Detail of the method is available on the NIMD website
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