In this paper, a portable and sensitive DNA quantification method based on personal glucose meters and isothermal circular strand-displacement polymerization reaction was developed. The capture DNA was fixed onto Streptavidin-MNBs.
Then target DNA (in red color) hybridized with the capture DNA and opened their loop parts, enabling a primer with DNA–invertase conjugation to attach on the stem part, which initiated a polymerization which replaced the target with the aid of the DNA polymerase I