carcinoma HT-29 [29]. Thus, some of these extracts may be toxic to invertebrates only. Moreover, sea
urchin egg bioassay has additionally been used as a model for evaluation of developmental toxicology,
as well as the brine shrimp bioassay based on the inhibition of hatching of cyst [30–32]. These bioassays
are useful tools to identify potential bio-ovicide chemical compounds.