The half maximal inhibitory concentration (ICso) determination
The IC50 value of extracts was determined at a concentratio of 500.0, 250.0, 125.0, 100.0, 25.0, 12.5 and 5.0 ug/ml. orlistat was used as a positive control. IC50 value was calculated by th following formula:
IC50=(50%-Law inh%)/(High inh%-Law inh%)*(High conc-Law conc)+Law conc
formula: where Low inh%/ High Inh% signify inhibition directly below/above 50% inhibition, and Low conc / High conc are the corresponding concentrations of extract.