Cassava cellulosic waste (Manihot esculenta,) obtained from starch processing was utilized in this work
for bio-ethanol production. The chemical composition such as insoluble carbohydrate, protein,
fibre and
residual starch content of the waste was determined. The substrate was then hydrolysed (acidic and
enzymatic), saccharified and fermented using a-amylase/HCl, amyloglucosidase and Saccharomyces
cerevisiae respectively. The results showed that combination of enzymatic and acid hydrolysis recovered
much of the starch and cellulose than when either of them was used. Dilute HCl was more helpful in
converting the cellulosic materials to reducing sugars. A total of 32.4% alcohol (2.7 g ethanol/15 g
cellulosic waste) was obtained which indicates that cassava wastes actually could be transformed to
chemicals for use as fuels, biochemicals, synthetic intermediates etc.
Cassava cellulosic waste (Manihot esculenta,) obtained from starch processing was utilized in this workfor bio-ethanol production. The chemical composition such as insoluble carbohydrate, protein,fibre andresidual starch content of the waste was determined. The substrate was then hydrolysed (acidic andenzymatic), saccharified and fermented using a-amylase/HCl, amyloglucosidase and Saccharomycescerevisiae respectively. The results showed that combination of enzymatic and acid hydrolysis recoveredmuch of the starch and cellulose than when either of them was used. Dilute HCl was more helpful inconverting the cellulosic materials to reducing sugars. A total of 32.4% alcohol (2.7 g ethanol/15 gcellulosic waste) was obtained which indicates that cassava wastes actually could be transformed tochemicals for use as fuels, biochemicals, synthetic intermediates etc.
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