Specificity of enzymes should be considered carefully, since antimicrobial activity is very sensitive to the environments and substrates. For an example, the activity. of lysozyme can be significantly affected by temperature and pH. In most cases, lysozyme is not effective against gmm-negitive bacteria. This is due to the coxplex cell wall structure of gmm-Negative bacteria and the Specificity of lysozyme for peptidoglycan.