Shoot tops from the field grown sugarcane somaclones
were collected every month up to 12th month and genomic
DNA was prepared for PCR analysis. Amplification studies
using smut specific primers revealed that among the somaclones tested, TC 906 and TC 922 did not revels the
presence of smut specific amplicon of 450 bp size up to
12 months of growth period. However in the susceptible
variety Co740, smut specific amplicon of 450 bp size was
detected at 4th month (Fig.2)