Another decrease in the Enterobacteriaceae load was measured in surface water biofilms, and no Enterobacteriaceae were detected in drinking water biofilms. The largest number of cefazolin-resistant Enterobacteriaceae was measured in biofilms from hospital wastewater (54±15%), followed by activated sludge biofilms (19±12%) and the plant discharge with 11±4.1%. The percentage resistance in surface water biofilms was 27±10%. The highest percentage of cefotaxime resistance was found in hospital wastewater biofilms (17±7.5%), followed by activated sludge biofilms (5.3±0.6%), and the plant discharge (1.9±0.4%). No cefotaxime-resistant Enterobacteriaceae were isolated from surface water biofilms.