Changes in the amounts of cellulose were compared among
these strains in agitation cultures prepared over 5 days on a
rotary shaker. The amount of cellulose produced by F2-2(pSACMCax/
k) cultured for 5 days was 77% of that produced by the
wild-type strain; however, the amount of cellulose produced by
F2-2 was very small (Fig. 1E). These results indicate that disruption
of cmcax causes a remarkable reduction in cellulose
production by G. xylinus.