Evaluation of Some Blood Parameters
Blood collection was carried out at the 4th week (28 and 30 days old) of the experiment. Three birds per
treatment were randomly selected and bled via wing veins using sterile 19 needles and syringes. About 5
ml of blood was collected into bottles containing ethylenediaminetetra acetic acid (EDTA). Blood
samples for serum biochemical studies were collected into vacuumed capillary tubes in order to determine
the blood cholesterol, triglyceride, high-density lipoprotein (HDL) and low-density lipoprotein (LDL)
levels. After coagulation, Blood samples were centrifuged at 2000 rpm, and then serum was collected and
stored at −20°C for later analysis. Blood cholesterol, triglyceride, HDL, and LDL levels were determined
spectrophotometrically by using commercial kits (Pars Azmoon Co., Teheran, Iran).