The causal fungus of leaf rust, P. triticina was isolated from infected
wheat leaves collected from the commercial wheat fields
and the Egyptian Wheat Rust Trap Nurseries during winter
growing seasons of 2012/13 and 2013/14.
Since the causal fungus of leaf rust is an obligate parasite,
rust isolates were maintained on living plants. For multiplication
of P. triticina uredospores, the collected samples were
transferred onto seedlings of the highly susceptible wheat
variety “Morocco” to obtain enough inoculum for further
investigations.