2.1.2. Eggs reared in the laboratory
Eggs collected in the same area and the same period
as the cuttlefish were incubated at the laboratory in
aquaria with an open circulation system. 4 pools of 25
eggs were dissected and embryos, yolk and eggshells
were separated for analysis. The embryos collected
from the eggs were about to hatch and were completely
formed but still attached to the yolk sack, which was not
completely resorbed. The rest of the eggs were maintained in aquaria until hatching, and the subsequent
25 juveniles hatching (ML=10±1 mm) were dissected
as previously described.