As we could not get available cell lines of rock bream through preliminary experiments, we screened out the most
sensitive cell line to the recombinant rock bream TNF-a by
analyzing luciferase activity in several fish cell lines, such as
Chinook salmon embryo (CHSE-214), grunt fin (GF), bluegill fin
(BF-2), and EPC cells, that were transfected with the NF-kB
reporting vector.