Prevalence of Total Bacteria and Staphylococcus aureus on Healthcare Workers’ Mobile Phone in Patient Department Aikchol Hospital, Chonburi Province. A total of 20 sample collected in November - December, 2013 using the swab test and the samples were cultured and examined by standard microbiological laboratory methods. The results showed amounts of the total bacteria (Aerobic plate count, APC) in mobile phone were 5.39 ± 3.15×10 cfu/cm2 - 3.09 ± 0.06×103 cfu/cm2, it was found that all samples mobile phone contaminated with bacteria (100 %). The amount of bacteria found in the samples, all samples of these phones is higher than the standard for microbiological indicator of surface hygiene in the hospital. According to the results, S. aureus contarmination in mobile phone example 14 were found 1 sample (5%) was conducted from 0.19 cfu/cm2