Dry extracts were dissolved in 600 ll water, of
which 500 ll were transferred to NMR tubes and mixed with
100 ll D2O, including 0.005% TMSP as an internal standard. 1H
NMR spectra of extracts were obtained on a Bruker Avance III
600, equipped with a TXI probe (Bruker Biospin, Rheinstetten, Germany)
operating at a 1H frequency of 600.13 MHz and 298 K. All
spectra were referenced to TMSP. One-dimensional (1D) 1H-NMR
spectra of aqueous extracts were acquired with a single 90 pulse,
a relaxation delay of 5 s, and 64 scans sweeping 12.7 ppm (offset
4.7 ppm) with 32 K data points.