All culture plates were allowed to dry for about 5 min and wells made using a sterile cork-borer (6 mm in diameter). These wells were respectively filled with 60 μL of the fungal extracts and control. After that, the plates were maintained at room temperature for 1 hour to allow the agents to diffuse into the agar medium before being incubated. The positive control was gentamicin (10 g/mL), while the negative control was DMSO (100 % v/v). After a 24-hour incubation period at 37 °C, the inhibition zone diameters (IZDs) were measured and values recorded.