Chemicals
1. Sulfuric acid 1.25%
2. Sodium hydroxide 1.25%
3. Ethyl alcohol 95%
Apparatus
1. Suction flask
2. Buchner funnel
3. Porcelain crucible
4. Hot plate
5. Furnace
6. Hot air oven
7. Desiccators
8. Whatman filter No.54
9. Analytical balance
Procedures
1. Determination the weight of filter paper by drying in hot air oven (105oC) for 1 h, then
cool in desiccators and record the weight. Store in the desiccators until use.
2. Determination the weight of crucible by heating in furnace (500oC) for 2 h, then cool
in desiccators and record the weight. Store in the desiccators until use.
3. Weight the sample (2-5 g) (defat before determining fiber) in a 500 ml beaker or Duran
bottle.
4. Add 200 ml of 1.25% sulfuric acid and then boil in the hot plate or water baht for 30
min. Cover the beaker with foil or loose cap duran bottle.