systematic characterisation of existing trans-selective lipase CAL-A was conducted and summarised in Table 2. The specificity and capacity of CAL-A was evaluated with the developed mathematical model, and CAL-A was found to have a selectivity factor 4.26 and to maximally remove 73.3% of total TFA at 46.5% hydrolysis degree. Obviously, the commercially available trans-selective enzyme is far from the least requisite of a selectivity factor (α > 58.21) that is capable to reduce TFA content in glycerides