Raw coffee were ground and screened through 250 lm
sieve to get a uniform texture. An accurately weighed
amount of sieved coffee (approximately 50 mg) was dissolved
in 25 ml of distilled water. The solution was stirred
for one hour using magnetic stirrer and heated gently to
remove caffeine easily from the solution. In addition the
solution was filtered by a glass filter to get rid of particle
from solution.