No shoot initiation from callus was observed on the
medium supplemented with 2,4-D even after 45 d and
60 d of culture, indicating the importance of cytokinins
for shoot induction. Among three cytokinins used, the
highest plant regeneration was observed on medium
supplemented with BAP, followed by medium
supplemented with kinetin and TDZ. No significant
morphological difference during shoot regeneration
among different shooting medium supplemented with
BAP and kinetin was observed. However, with only
TDZ supplemented medium, blackening of callus after
four weeks of culture was observed. Yellowing of shoots
on prolong culture (more than eight weeks) was more
prominent on TDZ supplemented medium as compared
to BAP and kinetin supplemented medium. Addition
of NAA in shooting medium resulted in increase in
regeneration frequency. The highest plantlet regeneration